Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR–Cas12f nuclease

Cas12f, also known as Cas14, is an exceptionally small type V-F CRISPR–Cas nuclease that is roughly half the size of comparable nucleases of this type. To reveal the mechanisms underlying substrate recognition and cleavage, we determined the cryo-EM structures of the Cas12f-sgRNA-target DNA and Cas1...

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Detalles Bibliográficos
Autores principales: Xiao, Renjian, Li, Zhuang, Wang, Shukun, Han, Ruijie, Chang, Leifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053092/
https://www.ncbi.nlm.nih.gov/pubmed/33764415
http://dx.doi.org/10.1093/nar/gkab179

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053092/
https://www.ncbi.nlm.nih.gov/pubmed/33764415
http://dx.doi.org/10.1093/nar/gkab179

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