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Structural basis for substrate recognition and cleavage by the dimerization-dependent CRISPR–Cas12f nuclease
Cas12f, also known as Cas14, is an exceptionally small type V-F CRISPR–Cas nuclease that is roughly half the size of comparable nucleases of this type. To reveal the mechanisms underlying substrate recognition and cleavage, we determined the cryo-EM structures of the Cas12f-sgRNA-target DNA and Cas1...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053092/ https://www.ncbi.nlm.nih.gov/pubmed/33764415 http://dx.doi.org/10.1093/nar/gkab179 |