Systematic in vitro specificity profiling reveals nicking defects in natural and engineered CRISPR–Cas9 variants

Cas9 is an RNA-guided endonuclease in the bacterial CRISPR–Cas immune system and a popular tool for genome editing. The commonly used Streptococcus pyogenes Cas9 (SpCas9) is relatively non-specific and prone to off-target genome editing. Other Cas9 orthologs and engineered variants of SpCas9 have be...

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Detalles Bibliográficos
Autores principales: Murugan, Karthik, Suresh, Shravanti K, Seetharam, Arun S, Severin, Andrew J, Sashital, Dipali G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053117/
https://www.ncbi.nlm.nih.gov/pubmed/33744974
http://dx.doi.org/10.1093/nar/gkab163

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8053117/
https://www.ncbi.nlm.nih.gov/pubmed/33744974
http://dx.doi.org/10.1093/nar/gkab163

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