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Amplification-free RNA detection with CRISPR–Cas13

CRISPR-based nucleic-acid detection is an emerging technology for molecular diagnostics. However, these methods generally require several hours and could cause amplification errors, due to the pre-amplification of target nucleic acids to enhance the detection sensitivity. Here, we developed a platfo...

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Detalles Bibliográficos
Autores principales: Shinoda, Hajime, Taguchi, Yuya, Nakagawa, Ryoya, Makino, Asami, Okazaki, Sae, Nakano, Masahiro, Muramoto, Yukiko, Takahashi, Chiharu, Takahashi, Ikuko, Ando, Jun, Noda, Takeshi, Nureki, Osamu, Nishimasu, Hiroshi, Watanabe, Rikiya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055673/
https://www.ncbi.nlm.nih.gov/pubmed/33875803
http://dx.doi.org/10.1038/s42003-021-02001-8