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Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy
Traumatic optic neuropathy (TON) refers to optic nerve damage caused by trauma, leading to partial or complete loss of vision. The primary treatment options, such as hormonal therapy and surgery, have limited efficacy. Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38), a functional end...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055942/ https://www.ncbi.nlm.nih.gov/pubmed/33889576 http://dx.doi.org/10.3389/fcell.2021.659783 |
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author | Wang, Tian Li, Yiming Guo, Miao Dong, Xue Liao, Mengyu Du, Mei Wang, Xiaohong Yin, Haifang Yan, Hua |
author_facet | Wang, Tian Li, Yiming Guo, Miao Dong, Xue Liao, Mengyu Du, Mei Wang, Xiaohong Yin, Haifang Yan, Hua |
author_sort | Wang, Tian |
collection | PubMed |
description | Traumatic optic neuropathy (TON) refers to optic nerve damage caused by trauma, leading to partial or complete loss of vision. The primary treatment options, such as hormonal therapy and surgery, have limited efficacy. Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38), a functional endogenous neuroprotective peptide, has emerged as a promising therapeutic agent. In this study, we used rat retinal ganglion cell (RGC) exosomes as nanosized vesicles for the delivery of PACAP38 loaded via the exosomal anchor peptide CP05 (EXO(PACAP38)). EXO(PACAP38) showed greater uptake efficiency in vitro and in vivo than PACAP38. The results showed that EXO(PACAP38) significantly enhanced the RGC survival rate and retinal nerve fiber layer thickness in a rat TON model. Moreover, EXO(PACAP38) significantly promoted axon regeneration and optic nerve function after injury. These findings indicate that EXO(PACAP38) can be used as a treatment option and may have therapeutic implications for patients with TON. |
format | Online Article Text |
id | pubmed-8055942 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80559422021-04-21 Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy Wang, Tian Li, Yiming Guo, Miao Dong, Xue Liao, Mengyu Du, Mei Wang, Xiaohong Yin, Haifang Yan, Hua Front Cell Dev Biol Cell and Developmental Biology Traumatic optic neuropathy (TON) refers to optic nerve damage caused by trauma, leading to partial or complete loss of vision. The primary treatment options, such as hormonal therapy and surgery, have limited efficacy. Pituitary adenylate cyclase-activating polypeptide 38 (PACAP38), a functional endogenous neuroprotective peptide, has emerged as a promising therapeutic agent. In this study, we used rat retinal ganglion cell (RGC) exosomes as nanosized vesicles for the delivery of PACAP38 loaded via the exosomal anchor peptide CP05 (EXO(PACAP38)). EXO(PACAP38) showed greater uptake efficiency in vitro and in vivo than PACAP38. The results showed that EXO(PACAP38) significantly enhanced the RGC survival rate and retinal nerve fiber layer thickness in a rat TON model. Moreover, EXO(PACAP38) significantly promoted axon regeneration and optic nerve function after injury. These findings indicate that EXO(PACAP38) can be used as a treatment option and may have therapeutic implications for patients with TON. Frontiers Media S.A. 2021-04-06 /pmc/articles/PMC8055942/ /pubmed/33889576 http://dx.doi.org/10.3389/fcell.2021.659783 Text en Copyright © 2021 Wang, Li, Guo, Dong, Liao, Du, Wang, Yin and Yan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Wang, Tian Li, Yiming Guo, Miao Dong, Xue Liao, Mengyu Du, Mei Wang, Xiaohong Yin, Haifang Yan, Hua Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title | Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title_full | Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title_fullStr | Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title_full_unstemmed | Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title_short | Exosome-Mediated Delivery of the Neuroprotective Peptide PACAP38 Promotes Retinal Ganglion Cell Survival and Axon Regeneration in Rats With Traumatic Optic Neuropathy |
title_sort | exosome-mediated delivery of the neuroprotective peptide pacap38 promotes retinal ganglion cell survival and axon regeneration in rats with traumatic optic neuropathy |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8055942/ https://www.ncbi.nlm.nih.gov/pubmed/33889576 http://dx.doi.org/10.3389/fcell.2021.659783 |
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