Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast

BACKGROUND: Translation is an important point of regulation in protein synthesis. However, there is a limited number of methods available to measure global translation activity in yeast. Recently, O-propargyl-puromycin (OPP) labelling has been established for mammalian cells, but unmodified yeasts a...

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Detalles Bibliográficos
Autores principales: Staudacher, Jennifer, Rebnegger, Corinna, Gasser, Brigitte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056590/
https://www.ncbi.nlm.nih.gov/pubmed/33879049
http://dx.doi.org/10.1186/s12866-021-02185-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056590/
https://www.ncbi.nlm.nih.gov/pubmed/33879049
http://dx.doi.org/10.1186/s12866-021-02185-3

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