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Treatment with surfactants enables quantification of translational activity by O-propargyl-puromycin labelling in yeast
BACKGROUND: Translation is an important point of regulation in protein synthesis. However, there is a limited number of methods available to measure global translation activity in yeast. Recently, O-propargyl-puromycin (OPP) labelling has been established for mammalian cells, but unmodified yeasts a...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056590/ https://www.ncbi.nlm.nih.gov/pubmed/33879049 http://dx.doi.org/10.1186/s12866-021-02185-3 |