Purification, Cloning, Functional Expression, Structure, and Characterization of a Thermostable β-Mannanase from Talaromyces trachyspermus B168 and Its Efficiency in Production of Mannooligosaccharides from Coffee Wastes

Highly thermostable β-mannanase, belonging to glycoside hydrolase family 5 subfamily 7, was purified from the culture supernatant of Talaromyces trachyspermus B168 and the cDNA of its transcript was cloned. The recombinant enzyme showed maximal activity at pH 4.5 and 85 °C. It retained more than 90...

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Detalles Bibliográficos
Autores principales: Suzuki, Kentaro, Michikawa, Mari, Sato, Haruna, Yuki, Masahiro, Kamino, Kei, Ogasawara, Wataru, Fushinobu, Shinya, Kaneko, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Applied Glycoscience 2018
Materias:
Regular Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056896/
https://www.ncbi.nlm.nih.gov/pubmed/34354508
http://dx.doi.org/10.5458/jag.jag.JAG-2017_018

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8056896/
https://www.ncbi.nlm.nih.gov/pubmed/34354508
http://dx.doi.org/10.5458/jag.jag.JAG-2017_018

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