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Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases

Kasugamycin, a well-known aminoglycoside antibiotic, has been used widely in agriculture and medicine to combat microbial pathogens by binding the ribosome to inhibit translation. Here, kasugamycin was discovered to be a competitive inhibitor of glycoside hydrolase family 18 (GH18) chitinases from t...

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Autores principales: Qi, Huitang, Jiang, Xi, Ding, Yi, Liu, Tian, Yang, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058351/
https://www.ncbi.nlm.nih.gov/pubmed/33898519
http://dx.doi.org/10.3389/fmolb.2021.640356
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author Qi, Huitang
Jiang, Xi
Ding, Yi
Liu, Tian
Yang, Qing
author_facet Qi, Huitang
Jiang, Xi
Ding, Yi
Liu, Tian
Yang, Qing
author_sort Qi, Huitang
collection PubMed
description Kasugamycin, a well-known aminoglycoside antibiotic, has been used widely in agriculture and medicine to combat microbial pathogens by binding the ribosome to inhibit translation. Here, kasugamycin was discovered to be a competitive inhibitor of glycoside hydrolase family 18 (GH18) chitinases from three different organisms (bacterium, insect and human). Results from tryptophan fluorescence spectroscopy and molecular docking revealed that kasugamycin binds to the substrate-binding clefts in a similar mode as the substrate. An electrostatic interaction between the amino group of kasugamycin and the carboxyl group of a conserved aspartate in GH18 chitinase (one of the catalytic triad residues) was found to be vital for the inhibitory activity. This paper not only reports new molecular targets of kasugamycin, but also expands our thinking about GH inhibitor design by using a scaffold unrelated to the substrate.
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spelling pubmed-80583512021-04-22 Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases Qi, Huitang Jiang, Xi Ding, Yi Liu, Tian Yang, Qing Front Mol Biosci Molecular Biosciences Kasugamycin, a well-known aminoglycoside antibiotic, has been used widely in agriculture and medicine to combat microbial pathogens by binding the ribosome to inhibit translation. Here, kasugamycin was discovered to be a competitive inhibitor of glycoside hydrolase family 18 (GH18) chitinases from three different organisms (bacterium, insect and human). Results from tryptophan fluorescence spectroscopy and molecular docking revealed that kasugamycin binds to the substrate-binding clefts in a similar mode as the substrate. An electrostatic interaction between the amino group of kasugamycin and the carboxyl group of a conserved aspartate in GH18 chitinase (one of the catalytic triad residues) was found to be vital for the inhibitory activity. This paper not only reports new molecular targets of kasugamycin, but also expands our thinking about GH inhibitor design by using a scaffold unrelated to the substrate. Frontiers Media S.A. 2021-04-07 /pmc/articles/PMC8058351/ /pubmed/33898519 http://dx.doi.org/10.3389/fmolb.2021.640356 Text en Copyright © 2021 Qi, Jiang, Ding, Liu and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Qi, Huitang
Jiang, Xi
Ding, Yi
Liu, Tian
Yang, Qing
Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title_full Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title_fullStr Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title_full_unstemmed Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title_short Discovery of Kasugamycin as a Potent Inhibitor of Glycoside Hydrolase Family 18 Chitinases
title_sort discovery of kasugamycin as a potent inhibitor of glycoside hydrolase family 18 chitinases
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8058351/
https://www.ncbi.nlm.nih.gov/pubmed/33898519
http://dx.doi.org/10.3389/fmolb.2021.640356
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