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Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms
Trans-4-hydroxy-l-proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans-4-hydroxy-l-proline is produced by the acidic hydrolysis of collagen,...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061225/ https://www.ncbi.nlm.nih.gov/pubmed/33882914 http://dx.doi.org/10.1186/s12934-021-01579-2 |
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author | Zhang, Zhenyu Liu, Pengfu Su, Weike Zhang, Huawei Xu, Wenqian Chu, Xiaohe |
author_facet | Zhang, Zhenyu Liu, Pengfu Su, Weike Zhang, Huawei Xu, Wenqian Chu, Xiaohe |
author_sort | Zhang, Zhenyu |
collection | PubMed |
description | Trans-4-hydroxy-l-proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans-4-hydroxy-l-proline is produced by the acidic hydrolysis of collagen, but this process has serious drawbacks, such as low productivity, a complex process and heavy environmental pollution. Presently, trans-4-hydroxy-l-proline is mainly produced via fermentative production by microorganisms. Some recently published advances in metabolic engineering have been used to effectively construct microbial cell factories that have improved the trans-4-hydroxy-l-proline biosynthetic pathway. To probe the potential of microorganisms for trans-4-hydroxy-l-proline production, new strategies and tools must be proposed. In this review, we provide a comprehensive understanding of trans-4-hydroxy-l-proline, including its biosynthetic pathway, proline hydroxylases and production by metabolic engineering, with a focus on improving its production. |
format | Online Article Text |
id | pubmed-8061225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-80612252021-04-22 Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms Zhang, Zhenyu Liu, Pengfu Su, Weike Zhang, Huawei Xu, Wenqian Chu, Xiaohe Microb Cell Fact Review Trans-4-hydroxy-l-proline is an important amino acid that is widely used in medicinal and industrial applications, particularly as a valuable chiral building block for the organic synthesis of pharmaceuticals. Traditionally, trans-4-hydroxy-l-proline is produced by the acidic hydrolysis of collagen, but this process has serious drawbacks, such as low productivity, a complex process and heavy environmental pollution. Presently, trans-4-hydroxy-l-proline is mainly produced via fermentative production by microorganisms. Some recently published advances in metabolic engineering have been used to effectively construct microbial cell factories that have improved the trans-4-hydroxy-l-proline biosynthetic pathway. To probe the potential of microorganisms for trans-4-hydroxy-l-proline production, new strategies and tools must be proposed. In this review, we provide a comprehensive understanding of trans-4-hydroxy-l-proline, including its biosynthetic pathway, proline hydroxylases and production by metabolic engineering, with a focus on improving its production. BioMed Central 2021-04-21 /pmc/articles/PMC8061225/ /pubmed/33882914 http://dx.doi.org/10.1186/s12934-021-01579-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Review Zhang, Zhenyu Liu, Pengfu Su, Weike Zhang, Huawei Xu, Wenqian Chu, Xiaohe Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title | Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title_full | Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title_fullStr | Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title_full_unstemmed | Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title_short | Metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
title_sort | metabolic engineering strategy for synthetizing trans-4-hydroxy-l-proline in microorganisms |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8061225/ https://www.ncbi.nlm.nih.gov/pubmed/33882914 http://dx.doi.org/10.1186/s12934-021-01579-2 |
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