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Protocol for measuring sphingolipid metabolism in budding yeast

Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceram...

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Detalles Bibliográficos
Autores principales: Ikeda, Atsuko, Hanaoka, Kazuki, Funato, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063906/
https://www.ncbi.nlm.nih.gov/pubmed/33912844
http://dx.doi.org/10.1016/j.xpro.2021.100412