Protocol for measuring sphingolipid metabolism in budding yeast

Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceram...

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Detalles Bibliográficos
Autores principales: Ikeda, Atsuko, Hanaoka, Kazuki, Funato, Kouichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063906/
https://www.ncbi.nlm.nih.gov/pubmed/33912844
http://dx.doi.org/10.1016/j.xpro.2021.100412
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author Ikeda, Atsuko
Hanaoka, Kazuki
Funato, Kouichi
author_facet Ikeda, Atsuko
Hanaoka, Kazuki
Funato, Kouichi
author_sort Ikeda, Atsuko
collection PubMed
description Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceramide and inositolphosphorylceramide (IPC) are classes of sphingolipids present in yeast and are metabolically labeled with radioactive precursors. This protocol for metabolic labeling can be used to investigate ceramide transport in an in vivo environment. For complete details on the use and execution of this protocol, please refer to Ikeda et al. (2020).
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spelling pubmed-80639062021-04-27 Protocol for measuring sphingolipid metabolism in budding yeast Ikeda, Atsuko Hanaoka, Kazuki Funato, Kouichi STAR Protoc Protocol Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceramide and inositolphosphorylceramide (IPC) are classes of sphingolipids present in yeast and are metabolically labeled with radioactive precursors. This protocol for metabolic labeling can be used to investigate ceramide transport in an in vivo environment. For complete details on the use and execution of this protocol, please refer to Ikeda et al. (2020). Elsevier 2021-04-10 /pmc/articles/PMC8063906/ /pubmed/33912844 http://dx.doi.org/10.1016/j.xpro.2021.100412 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ikeda, Atsuko
Hanaoka, Kazuki
Funato, Kouichi
Protocol for measuring sphingolipid metabolism in budding yeast
title Protocol for measuring sphingolipid metabolism in budding yeast
title_full Protocol for measuring sphingolipid metabolism in budding yeast
title_fullStr Protocol for measuring sphingolipid metabolism in budding yeast
title_full_unstemmed Protocol for measuring sphingolipid metabolism in budding yeast
title_short Protocol for measuring sphingolipid metabolism in budding yeast
title_sort protocol for measuring sphingolipid metabolism in budding yeast
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063906/
https://www.ncbi.nlm.nih.gov/pubmed/33912844
http://dx.doi.org/10.1016/j.xpro.2021.100412
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