Cargando…

Protocol for measuring sphingolipid metabolism in budding yeast

Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceram...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ikeda, Atsuko, Hanaoka, Kazuki, Funato, Kouichi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063906/ https://www.ncbi.nlm.nih.gov/pubmed/33912844 http://dx.doi.org/10.1016/j.xpro.2021.100412

Ejemplares similares

Membrane Contact Sites in Yeast: Control Hubs of Sphingolipid Homeostasis
por: Schlarmann, Philipp, et al.
Publicado: (2021)

Protocol to quantify palmitoylation of cysteines in budding yeast
por: Lei, Yuqing, et al.
Publicado: (2021)

The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast
por: Flor-Parra, Ignacio, et al.
Publicado: (2021)

Protocol for Titrating Gene Expression Levels in Budding Yeast
por: Qu, Yimiao, et al.
Publicado: (2020)

Protocol to purify and detect ubiquitinated phospholipids in budding yeast and human cell lines
por: Sakamaki, Jun-ichi, et al.
Publicado: (2022)

A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast
por: Novarina, Daniele, et al.
Publicado: (2022)

Incorporation of reporter genes into mitochondrial DNA in budding yeast
por: Rzepka, Magdalena, et al.
Publicado: (2022)

A conserved signaling network monitors delivery of sphingolipids to the plasma membrane in budding yeast
por: Clarke, Jesse, et al.
Publicado: (2017)

Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast
por: Challa, Kiran, et al.
Publicado: (2021)

Global mapping of translation initiation sites by TIS profiling in budding yeast
por: Hollerer, Ina, et al.
Publicado: (2020)

Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae
por: Yang, Emily J., et al.
Publicado: (2022)

Predicting nucleosome positioning using statistical equilibrium models in budding yeast
por: Kharerin, Hungyo, et al.
Publicado: (2022)

Protocol for establishing a protein interactome based on close physical proximity to a target protein within live budding yeast
por: Kolhe, Janhavi A., et al.
Publicado: (2023)

Analysis of the pheromone signaling pathway by RT-qPCR in the budding yeast Saccharomyces cerevisiae
por: Ramos-Alonso, Lucía, et al.
Publicado: (2022)

Using Taxol-sensitized budding yeast to investigate the effect of microtubule stabilization on anaphase onset
por: Bunning, Angela R., et al.
Publicado: (2023)

Utilizing chemically induced dimerization of FKBP to analyze endocytosis by live-cell imaging in budding yeast
por: Lamb, Andrew K., et al.
Publicado: (2022)

Sphingolipids in Childhood Asthma and Obesity (SOAP Study): A Protocol of a Cross-Sectional Study
por: Antonisamy, Belavendra, et al.
Publicado: (2023)

Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift
por: Alvarez-Vasquez, Fernando, et al.
Publicado: (2007)

Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast
por: Funato, Kouichi, et al.
Publicado: (2001)

A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9
por: Aguilar, Rhiannon R., et al.
Publicado: (2022)

Structural analysis of the GPI glycan
por: Nakano, Miyako, et al.
Publicado: (2021)

Long undecoded transcript isoform (LUTI) detection in meiotic budding yeast by direct RNA and transcript leader sequencing
por: Tresenrider, Amy, et al.
Publicado: (2022)

Measuring Replicative Life Span in the Budding Yeast
por: Steffen, Kristan K., et al.
Publicado: (2009)

Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
por: Jones, Andrew W., et al.
Publicado: (2020)

Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
por: Ryosaka, Makoto, et al.
Publicado: (2022)

Factors driving metabolic diversity in the budding yeast subphylum
por: Opulente, Dana A., et al.
Publicado: (2018)

Protocol for sleep analysis in the brain of genetically modified adult mice
por: Iwasaki, Kanako, et al.
Publicado: (2021)

Meiosis in budding yeast
por: Börner, G Valentin, et al.
Publicado: (2023)

Tricalbins Are Required for Non-vesicular Ceramide Transport at ER-Golgi Contacts and Modulate Lipid Droplet Biogenesis
por: Ikeda, Atsuko, et al.
Publicado: (2020)

Producing human ceramide-NS by metabolic engineering using yeast Saccharomyces cerevisiae
por: Murakami, Suguru, et al.
Publicado: (2015)

A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
por: Takao, Tomoka, et al.
Publicado: (2022)

Down-Regulating Sphingolipid Synthesis Increases Yeast Lifespan
por: Huang, Xinhe, et al.
Publicado: (2012)

Coordination of Rapid Sphingolipid Responses to Heat Stress in Yeast
por: Chen, Po-Wei, et al.
Publicado: (2013)

Sphingolipids and mitochondrial function, lessons learned from yeast
por: Spincemaille, Pieter, et al.
Publicado: (2014)

The Baltimore Urban Food Distribution (BUD) App: Study Protocol to Assess the Feasibility of a Food Systems Intervention
por: Gittelsohn, Joel, et al.
Publicado: (2022)

Selective autophagy in budding yeast
por: Suzuki, Kuninori
Publicado: (2013)

Actin and Endocytosis in Budding Yeast
por: Goode, Bruce L., et al.
Publicado: (2015)

Yeast Svf1 binds ceramides and contributes to sphingolipid metabolism at the ER cis-Golgi interface
por: Limar, Sergej, et al.
Publicado: (2023)

A Comprehensive Review: Sphingolipid Metabolism and Implications of Disruption in Sphingolipid Homeostasis
por: Quinville, Brianna M., et al.
Publicado: (2021)

Computational modeling of sphingolipid metabolism
por: Wronowska, Weronika, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_a8u8vc9mtsv2uggqgjiehglk2t