Detecting single cell interferon-beta production using a fluorescent reporter telomerase-immortalized human fibroblast cell line

Recent data suggest that cells respond to infection by upregulating the antiviral cytokine interferon-beta (IFN-ß) in a fraction of infected cells. Approaches are thus needed to study these responses on a single-cell level rather than bulk population. Here, we describe a protocol to analyze the IFN-...

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Detalles Bibliográficos
Autores principales: Hare, David N., Subapanditha, Minomi K., Mossman, Karen L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063907/
https://www.ncbi.nlm.nih.gov/pubmed/33912845
http://dx.doi.org/10.1016/j.xpro.2021.100436

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063907/
https://www.ncbi.nlm.nih.gov/pubmed/33912845
http://dx.doi.org/10.1016/j.xpro.2021.100436

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