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Detecting single cell interferon-beta production using a fluorescent reporter telomerase-immortalized human fibroblast cell line
Recent data suggest that cells respond to infection by upregulating the antiviral cytokine interferon-beta (IFN-ß) in a fraction of infected cells. Approaches are thus needed to study these responses on a single-cell level rather than bulk population. Here, we describe a protocol to analyze the IFN-...
Autores principales: | Hare, David N., Subapanditha, Minomi K., Mossman, Karen L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8063907/ https://www.ncbi.nlm.nih.gov/pubmed/33912845 http://dx.doi.org/10.1016/j.xpro.2021.100436 |
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