A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establ...

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Detalles Bibliográficos
Autores principales: He, Lingli, Yu, Wentao, Lu, Yi, Zhang, Wenxiang, Xu, Jinjin, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064019/
https://www.ncbi.nlm.nih.gov/pubmed/33912846
http://dx.doi.org/10.1016/j.xpro.2021.100445
Descripción
Sumario:Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020).

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