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A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establ...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064019/ https://www.ncbi.nlm.nih.gov/pubmed/33912846 http://dx.doi.org/10.1016/j.xpro.2021.100445 |
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author | He, Lingli Yu, Wentao Lu, Yi Zhang, Wenxiang Xu, Jinjin Zhang, Lei |
author_facet | He, Lingli Yu, Wentao Lu, Yi Zhang, Wenxiang Xu, Jinjin Zhang, Lei |
author_sort | He, Lingli |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020). |
format | Online Article Text |
id | pubmed-8064019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-80640192021-04-27 A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system He, Lingli Yu, Wentao Lu, Yi Zhang, Wenxiang Xu, Jinjin Zhang, Lei STAR Protoc Protocol Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020). Elsevier 2021-04-10 /pmc/articles/PMC8064019/ /pubmed/33912846 http://dx.doi.org/10.1016/j.xpro.2021.100445 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol He, Lingli Yu, Wentao Lu, Yi Zhang, Wenxiang Xu, Jinjin Zhang, Lei A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title | A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title_full | A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title_fullStr | A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title_full_unstemmed | A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title_short | A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
title_sort | protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064019/ https://www.ncbi.nlm.nih.gov/pubmed/33912846 http://dx.doi.org/10.1016/j.xpro.2021.100445 |
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