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A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establ...

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Detalles Bibliográficos
Autores principales: He, Lingli, Yu, Wentao, Lu, Yi, Zhang, Wenxiang, Xu, Jinjin, Zhang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064019/
https://www.ncbi.nlm.nih.gov/pubmed/33912846
http://dx.doi.org/10.1016/j.xpro.2021.100445
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author He, Lingli
Yu, Wentao
Lu, Yi
Zhang, Wenxiang
Xu, Jinjin
Zhang, Lei
author_facet He, Lingli
Yu, Wentao
Lu, Yi
Zhang, Wenxiang
Xu, Jinjin
Zhang, Lei
author_sort He, Lingli
collection PubMed
description Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020).
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spelling pubmed-80640192021-04-27 A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system He, Lingli Yu, Wentao Lu, Yi Zhang, Wenxiang Xu, Jinjin Zhang, Lei STAR Protoc Protocol Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer death worldwide. However, the pathogenesis of HCC is complicated, and the drugs used for HCC treatment are limited. The following protocol combines a genetically engineered mouse model (GEMM) with a sleeping beauty system to establish an in vivo liver tumorigenesis model. By using this model, the impact of genes of interest in liver tumorigenesis and progression can be studied. This model can also be applied to develop new therapeutic drugs for HCC. For complete details on the use and execution of this protocol, please refer to He et al. (2020). Elsevier 2021-04-10 /pmc/articles/PMC8064019/ /pubmed/33912846 http://dx.doi.org/10.1016/j.xpro.2021.100445 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
He, Lingli
Yu, Wentao
Lu, Yi
Zhang, Wenxiang
Xu, Jinjin
Zhang, Lei
A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title_full A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title_fullStr A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title_full_unstemmed A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title_short A protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
title_sort protocol for in vivo analysis of liver tumorigenesis in mice using sleeping beauty transposon system
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8064019/
https://www.ncbi.nlm.nih.gov/pubmed/33912846
http://dx.doi.org/10.1016/j.xpro.2021.100445
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