Cargando…

Purification and quantitative proteomic analysis of cell bodies and protrusions

Actin-rich protrusions are membrane extensions generated by actin polymerization that drive mesenchymal-like cell migration. Characterization of protrusions proteome is crucial for understanding their function. We present a complete step-by-step protocol based on microporous filter-based fractionati...

Descripción completa

Detalles Bibliográficos
Autores principales: Dermit, Maria, Mardakheh, Faraz K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065254/
https://www.ncbi.nlm.nih.gov/pubmed/33912849
http://dx.doi.org/10.1016/j.xpro.2021.100462