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Purification and quantitative proteomic analysis of cell bodies and protrusions
Actin-rich protrusions are membrane extensions generated by actin polymerization that drive mesenchymal-like cell migration. Characterization of protrusions proteome is crucial for understanding their function. We present a complete step-by-step protocol based on microporous filter-based fractionati...
Autores principales: | Dermit, Maria, Mardakheh, Faraz K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8065254/ https://www.ncbi.nlm.nih.gov/pubmed/33912849 http://dx.doi.org/10.1016/j.xpro.2021.100462 |
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