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Expression and Functional Analysis of the Argonaute Protein of Thermus thermophilus (TtAgo) in E. coli BL21(DE3)

The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts. In the present study, we expressed functional...

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Detalles Bibliográficos
Autores principales: Xing, Jiani, Ma, Lixia, Cheng, Xinzhen, Ma, Jinrong, Wang, Ruyu, Xu, Kun, Mymryk, Joe S., Zhang, Zhiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8067300/
https://www.ncbi.nlm.nih.gov/pubmed/33807395
http://dx.doi.org/10.3390/biom11040524