Cargando…

CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells

RNA interference (RNAi) designates sequence-specific mRNA degradation mediated by small RNAs generated from long double-stranded RNA (dsRNA) by RNase III Dicer. RNAi appears inactive in mammalian cells except for mouse oocytes, where high RNAi activity exists because of an N-terminally truncated Dic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Malik, Radek, Svoboda, Petr
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069103/ https://www.ncbi.nlm.nih.gov/pubmed/33918028 http://dx.doi.org/10.3390/genes12040540

Ejemplares similares

Production of small RNAs by mammalian Dicer
por: Svobodova, Eliska, et al.
Publicado: (2016)

Dicer Is Associated with Ribosomal DNA Chromatin in Mammalian Cells
por: Sinkkonen, Lasse, et al.
Publicado: (2010)

Main constraints for RNAi induced by expressed long dsRNA in mouse cells
por: Demeter, Tomas, et al.
Publicado: (2019)

Dicer structure and function: conserved and evolving features
por: Zapletal, David, et al.
Publicado: (2023)

Reporters Transiently Transfected into Mammalian Cells Are Highly Sensitive to Translational Repression Induced by dsRNA Expression
por: Nejepinska, Jana, et al.
Publicado: (2014)

dsRNA expression in the mouse elicits RNAi in oocytes and low adenosine deamination in somatic cells
por: Nejepinska, Jana, et al.
Publicado: (2012)

Structural and functional basis of mammalian microRNA biogenesis by Dicer
por: Zapletal, David, et al.
Publicado: (2022)

Over-expressed, N-terminally truncated BRAF is detected in the nucleus of cells with nuclear phosphorylated MEK and ERK
por: Hey, Fiona, et al.
Publicado: (2018)

Shortcomings of short hairpin RNA-based transgenic RNA interference in mouse oocytes
por: Sarnova, Lenka, et al.
Publicado: (2010)

NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
por: Chen, Yung-Fu, et al.
Publicado: (2007)

A N-terminal truncated intracellular isoform of matrix metalloproteinase-2 impairs contractility of mouse myocardium
por: Lovett, David H., et al.
Publicado: (2014)

Effects of Forced Expression of an NH(2)-terminal Truncated β-Catenin on Mouse Intestinal Epithelial Homeostasis
por: Wong, Melissa H., et al.
Publicado: (1998)

A Facile Method to Produce N-Terminally Truncated α-Synuclein
por: Thrush, Rebecca J., et al.
Publicado: (2022)

N-terminal VP1 Truncations Favor T = 1 Norovirus-Like Particles
por: Pogan, Ronja, et al.
Publicado: (2020)

The contribution of N-terminal truncated cMyBPC to in vivo cardiac function
por: Dominic, Katherine L., et al.
Publicado: (2023)

Effects of Dicer and Argonaute down-regulation on mRNA levels in human HEK293 cells
por: Schmitter, Daniela, et al.
Publicado: (2006)

N-terminally truncated POM121C inhibits HIV-1 replication
por: Saito, Hideki, et al.
Publicado: (2017)

Crystal structures of N-terminally truncated telomerase reverse transcriptase from fungi(‡)
por: Zhai, Liu-Tao, et al.
Publicado: (2021)

Identification of N-Terminally Truncated Derivatives of Insulin Analogs Formed in Pharmaceutical Formulations
por: Zielińska, Joanna, et al.
Publicado: (2018)

Low-Expressing Synucleinopathy Mouse Models Based on Oligomer-Forming Mutations and C-Terminal Truncation of α-Synuclein
por: Martinez Hernandez, Ana, et al.
Publicado: (2021)

Endogenous Mouse Dicer Is an Exclusively Cytoplasmic Protein
por: Much, Christian, et al.
Publicado: (2016)

N-Terminal Truncated UCH-L1 Prevents Parkinson's Disease Associated Damage
por: Kim, Hee-Jung, et al.
Publicado: (2014)

N-Terminal Truncated Intracellular Matrix Metalloproteinase-2 Induces Cardiomyocyte Hypertrophy, Inflammation and Systolic Heart Failure
por: Lovett, David H., et al.
Publicado: (2013)

CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
por: Cullot, Grégoire, et al.
Publicado: (2019)

The terminal loop region controls microRNA processing by Drosha and Dicer
por: Zhang, Xiaoxiao, et al.
Publicado: (2010)

N-terminally truncated Aβ4-x proteoforms and their relevance for Alzheimer’s pathophysiology
por: Rostagno, Agueda, et al.
Publicado: (2022)

Role of the Tau N-terminal region in microtubule stabilization revealed by new endogenous truncated forms
por: Derisbourg, Maxime, et al.
Publicado: (2015)

Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels
por: Webster, Jack M., et al.
Publicado: (2020)

Modification and Targeted Design of N-Terminal Truncates Derived from Brevinin with Improved Therapeutic Efficacy
por: He, Haoyang, et al.
Publicado: (2020)

Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes
por: Taborska, Eliska, et al.
Publicado: (2019)

The many faces of Dicer: the complexity of the mechanisms regulating Dicer gene expression and enzyme activities
por: Kurzynska-Kokorniak, Anna, et al.
Publicado: (2015)

Effects of truncations in the N‐ and C‐terminal domains of filensin on filament formation with phakinin in cell‐free conditions and cultured cells
por: Tashiro, Moe, et al.
Publicado: (2023)

N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro
por: Wei, Minxi, et al.
Publicado: (2018)

Up-regulation of alpha-smooth muscle actin in cardiomyocytes from non-hypertrophic and non-failing transgenic mouse hearts expressing N-terminal truncated cardiac troponin I()
por: Kern, Stephanie, et al.
Publicado: (2013)

Chromatin Changes in Dicer-Deficient Mouse Embryonic Stem Cells in Response to Retinoic Acid Induced Differentiation
por: Tennakoon, Jayantha B., et al.
Publicado: (2013)

Complexity in the regulation of Dicer expression: Dicer variant proteins are differentially expressed in epithelial and mesenchymal breast cancer cells and decreased during EMT
por: Hinkal, G W, et al.
Publicado: (2011)

Deep Sequencing Reveals Complex Spurious Transcription from Transiently Transfected Plasmids
por: Nejepinska, Jana, et al.
Publicado: (2012)

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
por: Fu, Yanfang, et al.
Publicado: (2014)

A truncated anti-CRISPR protein prevents spacer acquisition but not interference
por: Philippe, Cécile, et al.
Publicado: (2022)

N‐terminally and C‐terminally truncated forms of glucose‐dependent insulinotropic polypeptide are high‐affinity competitive antagonists of the human GIP receptor
por: Hansen, L S, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_ril5igg298hj0m3ak1151almjg