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Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards

SIMPLE SUMMARY: In the present report, we designed a reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) for the detection and quantification of the nervous necrosis virus (NNV), a fish virus of great importance in Mediterranean aquaculture. The advantage of this procedu...

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Detalles Bibliográficos
Autores principales: Olveira, José G., Souto, Sandra, Bandín, Isabel, Dopazo, Carlos P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069436/
https://www.ncbi.nlm.nih.gov/pubmed/33921441
http://dx.doi.org/10.3390/ani11041100