Cargando…

Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content

Various proteins or protein fractions reportedly positively affect gastrointestinal integrity and inflammation in diets providing >45% energy as fat. This study tested whether benefits were seen in diets providing 30% of energy as fat. Purified diets (PD) with isolated soy protein (ISP), dried wh...

Descripción completa

Detalles Bibliográficos
Autores principales: Price, Tara R., Baskaran, Sangeetha A., Moncada, Kristin L., Minamoto, Yasushi, Klemashevich, Cory, Jayuraman, Arul, Sucholdoski, Jan S., Tedeschi, Luis O., Steiner, Jörg M., Pillai, Suresh D., Walzem, Rosemary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069602/
https://www.ncbi.nlm.nih.gov/pubmed/33920187
http://dx.doi.org/10.3390/nu13041251
_version_ 1783683275670159360
author Price, Tara R.
Baskaran, Sangeetha A.
Moncada, Kristin L.
Minamoto, Yasushi
Klemashevich, Cory
Jayuraman, Arul
Sucholdoski, Jan S.
Tedeschi, Luis O.
Steiner, Jörg M.
Pillai, Suresh D.
Walzem, Rosemary L.
author_facet Price, Tara R.
Baskaran, Sangeetha A.
Moncada, Kristin L.
Minamoto, Yasushi
Klemashevich, Cory
Jayuraman, Arul
Sucholdoski, Jan S.
Tedeschi, Luis O.
Steiner, Jörg M.
Pillai, Suresh D.
Walzem, Rosemary L.
author_sort Price, Tara R.
collection PubMed
description Various proteins or protein fractions reportedly positively affect gastrointestinal integrity and inflammation in diets providing >45% energy as fat. This study tested whether benefits were seen in diets providing 30% of energy as fat. Purified diets (PD) with isolated soy protein (ISP), dried whole milk powder (DWMP), milk fat globule membrane (MFGM), or milk protein concentrate (MPC) as protein sources were fed to C57BL/6J mice (n = 15/diet group) for 13 weeks. MFGM-fed mice were heaviest (p < 0.005) but remained within breeder norms. Growth rates and gut motility were similar for all PD-fed mice. FITC-dextran assessed gut permeability was lowest in DWMP and MFGM (p = 0.054); overall, plasma endotoxin and unprovoked circulating cytokines indicated a non-inflammatory state for all PD-fed mice. Despite differences in cecal butyrate and intestinal gene expression, all PDs supported gastrointestinal health. Whole milk provided more positive effects compared to its fractions. However, ISP-fed mice showed a >370%, (p < 0.006) increase in colonic myeloperoxidase activity indicative of tissue neutrophil infiltration. Surprisingly, FITC-dextran and endotoxin outcomes were many folds better in PD-fed mice than mice (strain, vendor, age and sex matched) fed a “chow-type” nutritionally adequate non-PD. Additional variables within a diet’s matrix appear to affect routine indicators or gastrointestinal health.
format Online
Article
Text
id pubmed-8069602
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-80696022021-04-26 Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content Price, Tara R. Baskaran, Sangeetha A. Moncada, Kristin L. Minamoto, Yasushi Klemashevich, Cory Jayuraman, Arul Sucholdoski, Jan S. Tedeschi, Luis O. Steiner, Jörg M. Pillai, Suresh D. Walzem, Rosemary L. Nutrients Article Various proteins or protein fractions reportedly positively affect gastrointestinal integrity and inflammation in diets providing >45% energy as fat. This study tested whether benefits were seen in diets providing 30% of energy as fat. Purified diets (PD) with isolated soy protein (ISP), dried whole milk powder (DWMP), milk fat globule membrane (MFGM), or milk protein concentrate (MPC) as protein sources were fed to C57BL/6J mice (n = 15/diet group) for 13 weeks. MFGM-fed mice were heaviest (p < 0.005) but remained within breeder norms. Growth rates and gut motility were similar for all PD-fed mice. FITC-dextran assessed gut permeability was lowest in DWMP and MFGM (p = 0.054); overall, plasma endotoxin and unprovoked circulating cytokines indicated a non-inflammatory state for all PD-fed mice. Despite differences in cecal butyrate and intestinal gene expression, all PDs supported gastrointestinal health. Whole milk provided more positive effects compared to its fractions. However, ISP-fed mice showed a >370%, (p < 0.006) increase in colonic myeloperoxidase activity indicative of tissue neutrophil infiltration. Surprisingly, FITC-dextran and endotoxin outcomes were many folds better in PD-fed mice than mice (strain, vendor, age and sex matched) fed a “chow-type” nutritionally adequate non-PD. Additional variables within a diet’s matrix appear to affect routine indicators or gastrointestinal health. MDPI 2021-04-10 /pmc/articles/PMC8069602/ /pubmed/33920187 http://dx.doi.org/10.3390/nu13041251 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Price, Tara R.
Baskaran, Sangeetha A.
Moncada, Kristin L.
Minamoto, Yasushi
Klemashevich, Cory
Jayuraman, Arul
Sucholdoski, Jan S.
Tedeschi, Luis O.
Steiner, Jörg M.
Pillai, Suresh D.
Walzem, Rosemary L.
Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title_full Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title_fullStr Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title_full_unstemmed Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title_short Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content
title_sort whole and isolated protein fractions differentially affect gastrointestinal integrity markers in c57bl/6 mice fed diets with a moderate-fat content
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8069602/
https://www.ncbi.nlm.nih.gov/pubmed/33920187
http://dx.doi.org/10.3390/nu13041251
work_keys_str_mv AT pricetarar wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT baskaransangeethaa wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT moncadakristinl wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT minamotoyasushi wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT klemashevichcory wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT jayuramanarul wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT sucholdoskijans wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT tedeschiluiso wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT steinerjorgm wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT pillaisureshd wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent
AT walzemrosemaryl wholeandisolatedproteinfractionsdifferentiallyaffectgastrointestinalintegritymarkersinc57bl6micefeddietswithamoderatefatcontent