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Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071370/ https://www.ncbi.nlm.nih.gov/pubmed/33921012 http://dx.doi.org/10.3390/molecules26082291 |
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author | Rajiv, Chongtham Roy, Subhra Saikat Tamreihao, K. Kshetri, Pintubala Singh, Thangjam Surchandra Sanjita Devi, Haobijam Sharma, Susheel Kumar Ansari, Meraj Alam Devi, Elangbam Diana Devi, Asem Kajal Langamba, Pangambam Singh, Heikham Naresh Akoijam, Romila Tania, Chongtham Sonia, Chongtham |
author_facet | Rajiv, Chongtham Roy, Subhra Saikat Tamreihao, K. Kshetri, Pintubala Singh, Thangjam Surchandra Sanjita Devi, Haobijam Sharma, Susheel Kumar Ansari, Meraj Alam Devi, Elangbam Diana Devi, Asem Kajal Langamba, Pangambam Singh, Heikham Naresh Akoijam, Romila Tania, Chongtham Sonia, Chongtham |
author_sort | Rajiv, Chongtham |
collection | PubMed |
description | Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of this study is to investigate the antioxidant and anticancer activities of Jussiaea repens L., a wild edible flora found in Manipur, India. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) assay and DNA-nicking assay. The anticancer activity was tested using five cancer lines viz., SKOV3 cells (ovarian), HeLa (cervical), MDA-MB-231 (breast), PANC-1 (pancreatic), and PC3 (prostate). The toxicity, developmental effect, antiproliferative activity was further tested using zebrafish embryos. The methanolic plant extract had higher polyphenol content than flavonoids. The in vitro study demonstrated a promising antioxidant capacity and DNA protection ability of this plant. The extract also showed cytotoxic activity against SKOV3, HeLa, MDA-MB-23, and PANC-1 cancer cell lines. The in vivo studies on zebrafish embryos demonstrated the extract’s ability to suppress the developmental process and elicited more cytotoxicity to cancer cells than developing zebrafish embryos. Moreover, the in vivo studies on zebrafish embryos also indicated the antiproliferative activity of J. repens L. extract. |
format | Online Article Text |
id | pubmed-8071370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80713702021-04-26 Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model Rajiv, Chongtham Roy, Subhra Saikat Tamreihao, K. Kshetri, Pintubala Singh, Thangjam Surchandra Sanjita Devi, Haobijam Sharma, Susheel Kumar Ansari, Meraj Alam Devi, Elangbam Diana Devi, Asem Kajal Langamba, Pangambam Singh, Heikham Naresh Akoijam, Romila Tania, Chongtham Sonia, Chongtham Molecules Article Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of this study is to investigate the antioxidant and anticancer activities of Jussiaea repens L., a wild edible flora found in Manipur, India. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) assay and DNA-nicking assay. The anticancer activity was tested using five cancer lines viz., SKOV3 cells (ovarian), HeLa (cervical), MDA-MB-231 (breast), PANC-1 (pancreatic), and PC3 (prostate). The toxicity, developmental effect, antiproliferative activity was further tested using zebrafish embryos. The methanolic plant extract had higher polyphenol content than flavonoids. The in vitro study demonstrated a promising antioxidant capacity and DNA protection ability of this plant. The extract also showed cytotoxic activity against SKOV3, HeLa, MDA-MB-23, and PANC-1 cancer cell lines. The in vivo studies on zebrafish embryos demonstrated the extract’s ability to suppress the developmental process and elicited more cytotoxicity to cancer cells than developing zebrafish embryos. Moreover, the in vivo studies on zebrafish embryos also indicated the antiproliferative activity of J. repens L. extract. MDPI 2021-04-15 /pmc/articles/PMC8071370/ /pubmed/33921012 http://dx.doi.org/10.3390/molecules26082291 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rajiv, Chongtham Roy, Subhra Saikat Tamreihao, K. Kshetri, Pintubala Singh, Thangjam Surchandra Sanjita Devi, Haobijam Sharma, Susheel Kumar Ansari, Meraj Alam Devi, Elangbam Diana Devi, Asem Kajal Langamba, Pangambam Singh, Heikham Naresh Akoijam, Romila Tania, Chongtham Sonia, Chongtham Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title | Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title_full | Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title_fullStr | Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title_full_unstemmed | Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title_short | Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model |
title_sort | anticarcinogenic and antioxidant action of an edible aquatic flora jussiaea repens l. using in vitro bioassays and in vivo zebrafish model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071370/ https://www.ncbi.nlm.nih.gov/pubmed/33921012 http://dx.doi.org/10.3390/molecules26082291 |
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