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Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model

Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of...

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Autores principales: Rajiv, Chongtham, Roy, Subhra Saikat, Tamreihao, K., Kshetri, Pintubala, Singh, Thangjam Surchandra, Sanjita Devi, Haobijam, Sharma, Susheel Kumar, Ansari, Meraj Alam, Devi, Elangbam Diana, Devi, Asem Kajal, Langamba, Pangambam, Singh, Heikham Naresh, Akoijam, Romila, Tania, Chongtham, Sonia, Chongtham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071370/
https://www.ncbi.nlm.nih.gov/pubmed/33921012
http://dx.doi.org/10.3390/molecules26082291
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author Rajiv, Chongtham
Roy, Subhra Saikat
Tamreihao, K.
Kshetri, Pintubala
Singh, Thangjam Surchandra
Sanjita Devi, Haobijam
Sharma, Susheel Kumar
Ansari, Meraj Alam
Devi, Elangbam Diana
Devi, Asem Kajal
Langamba, Pangambam
Singh, Heikham Naresh
Akoijam, Romila
Tania, Chongtham
Sonia, Chongtham
author_facet Rajiv, Chongtham
Roy, Subhra Saikat
Tamreihao, K.
Kshetri, Pintubala
Singh, Thangjam Surchandra
Sanjita Devi, Haobijam
Sharma, Susheel Kumar
Ansari, Meraj Alam
Devi, Elangbam Diana
Devi, Asem Kajal
Langamba, Pangambam
Singh, Heikham Naresh
Akoijam, Romila
Tania, Chongtham
Sonia, Chongtham
author_sort Rajiv, Chongtham
collection PubMed
description Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of this study is to investigate the antioxidant and anticancer activities of Jussiaea repens L., a wild edible flora found in Manipur, India. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) assay and DNA-nicking assay. The anticancer activity was tested using five cancer lines viz., SKOV3 cells (ovarian), HeLa (cervical), MDA-MB-231 (breast), PANC-1 (pancreatic), and PC3 (prostate). The toxicity, developmental effect, antiproliferative activity was further tested using zebrafish embryos. The methanolic plant extract had higher polyphenol content than flavonoids. The in vitro study demonstrated a promising antioxidant capacity and DNA protection ability of this plant. The extract also showed cytotoxic activity against SKOV3, HeLa, MDA-MB-23, and PANC-1 cancer cell lines. The in vivo studies on zebrafish embryos demonstrated the extract’s ability to suppress the developmental process and elicited more cytotoxicity to cancer cells than developing zebrafish embryos. Moreover, the in vivo studies on zebrafish embryos also indicated the antiproliferative activity of J. repens L. extract.
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spelling pubmed-80713702021-04-26 Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model Rajiv, Chongtham Roy, Subhra Saikat Tamreihao, K. Kshetri, Pintubala Singh, Thangjam Surchandra Sanjita Devi, Haobijam Sharma, Susheel Kumar Ansari, Meraj Alam Devi, Elangbam Diana Devi, Asem Kajal Langamba, Pangambam Singh, Heikham Naresh Akoijam, Romila Tania, Chongtham Sonia, Chongtham Molecules Article Oxidative stress is the major cause of many health conditions, and regular consumption of antioxidants helped to encounter and prevent such oxidative stress-related diseases. Due to safety concerns over long-term uses of synthetic antioxidants, natural antioxidants are more preferred. The purpose of this study is to investigate the antioxidant and anticancer activities of Jussiaea repens L., a wild edible flora found in Manipur, India. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Ferric reducing antioxidant power (FRAP) assay and DNA-nicking assay. The anticancer activity was tested using five cancer lines viz., SKOV3 cells (ovarian), HeLa (cervical), MDA-MB-231 (breast), PANC-1 (pancreatic), and PC3 (prostate). The toxicity, developmental effect, antiproliferative activity was further tested using zebrafish embryos. The methanolic plant extract had higher polyphenol content than flavonoids. The in vitro study demonstrated a promising antioxidant capacity and DNA protection ability of this plant. The extract also showed cytotoxic activity against SKOV3, HeLa, MDA-MB-23, and PANC-1 cancer cell lines. The in vivo studies on zebrafish embryos demonstrated the extract’s ability to suppress the developmental process and elicited more cytotoxicity to cancer cells than developing zebrafish embryos. Moreover, the in vivo studies on zebrafish embryos also indicated the antiproliferative activity of J. repens L. extract. MDPI 2021-04-15 /pmc/articles/PMC8071370/ /pubmed/33921012 http://dx.doi.org/10.3390/molecules26082291 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rajiv, Chongtham
Roy, Subhra Saikat
Tamreihao, K.
Kshetri, Pintubala
Singh, Thangjam Surchandra
Sanjita Devi, Haobijam
Sharma, Susheel Kumar
Ansari, Meraj Alam
Devi, Elangbam Diana
Devi, Asem Kajal
Langamba, Pangambam
Singh, Heikham Naresh
Akoijam, Romila
Tania, Chongtham
Sonia, Chongtham
Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title_full Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title_fullStr Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title_full_unstemmed Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title_short Anticarcinogenic and Antioxidant Action of an Edible Aquatic Flora Jussiaea repens L. Using In Vitro Bioassays and In Vivo Zebrafish Model
title_sort anticarcinogenic and antioxidant action of an edible aquatic flora jussiaea repens l. using in vitro bioassays and in vivo zebrafish model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8071370/
https://www.ncbi.nlm.nih.gov/pubmed/33921012
http://dx.doi.org/10.3390/molecules26082291
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