Large-Scale Preparation of Highly Stable Recombinant Human Acidic Fibroblast Growth Factor in Escherichia coli BL21(DE3) plysS Strain

In this study, the optimum human aFGF gene encoding haFGF(135) was cloned in pET3c and transferred to Escherichia coli BL21(DE3) plysS. To enhance the yield of fermentation and the expression level of the target protein, the fermentation parameters, including temperature, pH, dissolved oxygen, gluco...

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Detalles Bibliográficos
Autores principales: Yu, Bingjie, Sun, Wenzhe, Huang, Zhen, Sun, Gang, Li, Le, Gu, Jiawei, Zheng, Mengying, Li, Xiaokun, Chun, ChangJu, Hui, Qi, Wang, Xiaojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072344/
https://www.ncbi.nlm.nih.gov/pubmed/33912546
http://dx.doi.org/10.3389/fbioe.2021.641505

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8072344/
https://www.ncbi.nlm.nih.gov/pubmed/33912546
http://dx.doi.org/10.3389/fbioe.2021.641505

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