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A GC-MS/Single-Cell Method to Evaluate Membrane Transporter Substrate Specificity and Signaling

Amino acid transporters play a vital role in metabolism and nutrient signaling pathways. Typically, transport activity is investigated using single substrates and competing amounts of other amino acids. We used GC-MS and LC-MS for metabolic screening of Xenopus laevis oocytes expressing various huma...

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Detalles Bibliográficos
Autores principales: Fairweather, Stephen J., Okada, Shoko, Gauthier-Coles, Gregory, Javed, Kiran, Bröer, Angelika, Bröer, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8076599/
https://www.ncbi.nlm.nih.gov/pubmed/33928121
http://dx.doi.org/10.3389/fmolb.2021.646574