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High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells

Macrophages are pivotal effectors of host immunity and regulators of tissue homeostasis. Understanding of human macrophage biology has been hampered by the lack of reliable and scalable models for cellular and genetic studies. Human induced pluripotent stem cell (hiPSC)-derived monocytes and macroph...

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Autores principales: Cui, Di, Franz, Alexandra, Fillon, Sophie A., Jannetti, Linda, Isambert, Timo, Fundel-Clemens, Katrin, Huber, Heinrich J., Viollet, Coralie, Ghanem, Alexander, Niwa, Akira, Weigle, Bernd, Pflanz, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080307/
https://www.ncbi.nlm.nih.gov/pubmed/33937256
http://dx.doi.org/10.3389/fcell.2021.656867
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author Cui, Di
Franz, Alexandra
Fillon, Sophie A.
Jannetti, Linda
Isambert, Timo
Fundel-Clemens, Katrin
Huber, Heinrich J.
Viollet, Coralie
Ghanem, Alexander
Niwa, Akira
Weigle, Bernd
Pflanz, Stefan
author_facet Cui, Di
Franz, Alexandra
Fillon, Sophie A.
Jannetti, Linda
Isambert, Timo
Fundel-Clemens, Katrin
Huber, Heinrich J.
Viollet, Coralie
Ghanem, Alexander
Niwa, Akira
Weigle, Bernd
Pflanz, Stefan
author_sort Cui, Di
collection PubMed
description Macrophages are pivotal effectors of host immunity and regulators of tissue homeostasis. Understanding of human macrophage biology has been hampered by the lack of reliable and scalable models for cellular and genetic studies. Human induced pluripotent stem cell (hiPSC)-derived monocytes and macrophages, as an unlimited source of subject genotype-specific cells, will undoubtedly play an important role in advancing our understanding of macrophage biology and implication in human diseases. In this study, we present a fully optimized differentiation protocol of hiPSC-derived monocytes and granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF). We present characterization of iPSC-derived myeloid lineage cells at phenotypic, functional, and transcriptomic levels, in comparison with corresponding subsets of peripheral blood-derived cells. We also highlight the application of hiPSC-derived monocytes and macrophages as a gene-editing platform for functional validation in research and drug screening, and the study also provides a reference for cell therapies.
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spelling pubmed-80803072021-04-29 High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells Cui, Di Franz, Alexandra Fillon, Sophie A. Jannetti, Linda Isambert, Timo Fundel-Clemens, Katrin Huber, Heinrich J. Viollet, Coralie Ghanem, Alexander Niwa, Akira Weigle, Bernd Pflanz, Stefan Front Cell Dev Biol Cell and Developmental Biology Macrophages are pivotal effectors of host immunity and regulators of tissue homeostasis. Understanding of human macrophage biology has been hampered by the lack of reliable and scalable models for cellular and genetic studies. Human induced pluripotent stem cell (hiPSC)-derived monocytes and macrophages, as an unlimited source of subject genotype-specific cells, will undoubtedly play an important role in advancing our understanding of macrophage biology and implication in human diseases. In this study, we present a fully optimized differentiation protocol of hiPSC-derived monocytes and granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage colony-stimulating factor (M-CSF). We present characterization of iPSC-derived myeloid lineage cells at phenotypic, functional, and transcriptomic levels, in comparison with corresponding subsets of peripheral blood-derived cells. We also highlight the application of hiPSC-derived monocytes and macrophages as a gene-editing platform for functional validation in research and drug screening, and the study also provides a reference for cell therapies. Frontiers Media S.A. 2021-04-13 /pmc/articles/PMC8080307/ /pubmed/33937256 http://dx.doi.org/10.3389/fcell.2021.656867 Text en Copyright © 2021 Cui, Franz, Fillon, Jannetti, Isambert, Fundel-Clemens, Huber, Viollet, Ghanem, Niwa, Weigle and Pflanz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Cui, Di
Franz, Alexandra
Fillon, Sophie A.
Jannetti, Linda
Isambert, Timo
Fundel-Clemens, Katrin
Huber, Heinrich J.
Viollet, Coralie
Ghanem, Alexander
Niwa, Akira
Weigle, Bernd
Pflanz, Stefan
High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title_full High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title_fullStr High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title_full_unstemmed High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title_short High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells
title_sort high-yield human induced pluripotent stem cell-derived monocytes and macrophages are functionally comparable with primary cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080307/
https://www.ncbi.nlm.nih.gov/pubmed/33937256
http://dx.doi.org/10.3389/fcell.2021.656867
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