Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts

Genetic manipulation of neural precursor cells is an important tool to study mechanisms underlying proliferation, fate specification, and neuron formation. The CRISPR/Cas9 system enables efficient genome editing but requires the clonal expansion of cells containing the desired mutation. Here, we des...

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Detalles Bibliográficos
Autores principales: Zocher, Sara, Kempermann, Gerd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080521/
https://www.ncbi.nlm.nih.gov/pubmed/33948565
http://dx.doi.org/10.1016/j.xpro.2021.100472

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080521/
https://www.ncbi.nlm.nih.gov/pubmed/33948565
http://dx.doi.org/10.1016/j.xpro.2021.100472

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