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Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts

Genetic manipulation of neural precursor cells is an important tool to study mechanisms underlying proliferation, fate specification, and neuron formation. The CRISPR/Cas9 system enables efficient genome editing but requires the clonal expansion of cells containing the desired mutation. Here, we des...

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Detalles Bibliográficos
Autores principales:	Zocher, Sara, Kempermann, Gerd
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080521/ https://www.ncbi.nlm.nih.gov/pubmed/33948565 http://dx.doi.org/10.1016/j.xpro.2021.100472

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