Cargando…

Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts

Genetic manipulation of neural precursor cells is an important tool to study mechanisms underlying proliferation, fate specification, and neuron formation. The CRISPR/Cas9 system enables efficient genome editing but requires the clonal expansion of cells containing the desired mutation. Here, we des...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zocher, Sara, Kempermann, Gerd
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080521/ https://www.ncbi.nlm.nih.gov/pubmed/33948565 http://dx.doi.org/10.1016/j.xpro.2021.100472

Ejemplares similares

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
por: Huber, Anne, et al.
Publicado: (2023)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
por: Giuliano, Christopher J., et al.
Publicado: (2019)

Protocol for generating monoclonal CRISPR-Cas9-mediated knockout cell lines using RNPs and lipofection in HNSCC cells
por: Geyer, Fabian, et al.
Publicado: (2023)

One-step CRISPR-Cas9 protocol for the generation of plug & play conditional knockouts in Drosophila melanogaster
por: Yu, Joyce J.S., et al.
Publicado: (2021)

L-lactate exerts a pro-proliferative effect on adult hippocampal precursor cells in vitro
por: Pötzsch, Alexandra, et al.
Publicado: (2021)

Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
por: Zhong, Aaron, et al.
Publicado: (2020)

A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system
por: Aouida, Mustapha, et al.
Publicado: (2022)

Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
por: Chan, Dedrick Kok Hong, et al.
Publicado: (2023)

Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells
por: Morrow, Christopher S., et al.
Publicado: (2021)

CRISPR/Cas9-mediated generation of a Plac8 knockout mouse model
por: Lee, HyunJeong, et al.
Publicado: (2018)

A Fast and Cost-Effective Genotyping Method for CRISPR-Cas9-Generated Mutant Rice Lines
por: Ablazov, Abdugaffor, et al.
Publicado: (2023)

Robust CRISPR/Cas9 Genome Editing of the HUDEP-2 Erythroid Precursor Line Using Plasmids and Single-Stranded Oligonucleotide Donors
por: Moir-Meyer, Gemma, et al.
Publicado: (2018)

An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system
por: Lu, Xibin, et al.
Publicado: (2020)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system
por: Li, Hsing-Han, et al.
Publicado: (2021)

Generation of Gene-Knockout Mongolian Gerbils via CRISPR/Cas9 System
por: Wang, Yan, et al.
Publicado: (2020)

One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporation
por: Flumens, Donovan, et al.
Publicado: (2023)

Generation of a homozygous CIITA knockout iPS cell line using the CRISPR-Cas9 system
por: Romano, Elena, et al.
Publicado: (2021)

Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology
por: Wang, Xiao, et al.
Publicado: (2021)

Optimized CRISPR/Cas9-mediated single nucleotide mutation in adherent cancer cell lines
por: Gao, Ping, et al.
Publicado: (2021)

Optimized protocol to create deletion in adherent cell lines using CRISPR/Cas9 system
por: Farooq, Umer, et al.
Publicado: (2021)

An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines in Bombyx mori
por: Brady, Daniel, et al.
Publicado: (2020)

CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells
por: Ghetti, Sabrina, et al.
Publicado: (2021)

Generation of epitope tag knock-in mice with CRISPR-Cas9 to study the function of endogenous proteins
por: Zhang, Zhao
Publicado: (2023)

Exploiting CRISPR/Cas9 to engineer precise segmental deletions in mouse embryonic stem cells
por: Elango, Rajula, et al.
Publicado: (2022)

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation
por: Gim, Gyeong-Min, et al.
Publicado: (2023)

CRISPR/Cas9-mediated gene knockout in the mouse brain using in utero electroporation
por: Shinmyo, Yohei, et al.
Publicado: (2016)

CRISPR/Cas9 RET Gene Knockout in Medullary Thyroid Carcinoma Cell-lines: Optimization and Validation
por: Zarif-Yeganeh, Marjan, et al.
Publicado: (2022)

CRISPR-Cas9 screen in human embryonic stem cells to identify genes required for neural differentiation
por: Qi, Shutao, et al.
Publicado: (2022)

Generation of mouse models carrying B cell restricted single or multiplexed loss-of-function mutations through CRISPR-Cas9 gene editing
por: ten Hacken, Elisa, et al.
Publicado: (2023)

Generating CRISPR/Cas9-Derived Mutant Mice by Zygote Cytoplasmic Injection Using an Automatic Microinjector
por: Doe, Brendan, et al.
Publicado: (2018)

Generation of Casp8(FL122/123GG) Mice Using CRISPR-Cas9 Technology
por: Pelletier, Stephane, et al.
Publicado: (2020)

CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice
por: Cassidy, Annelise, et al.
Publicado: (2023)

Optimized protocols for efficient gene editing in mouse hepatocytes in vivo using CRISPR-Cas9 technology
por: Chen, Yanhao, et al.
Publicado: (2021)

Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9
por: Biegler, Matthew T., et al.
Publicado: (2022)

CRISPR/Cas9-mediated tissue-specific knockout and cDNA rescue using sgRNAs that target exon-intron junctions in Drosophila melanogaster
por: Chilian, Madison, et al.
Publicado: (2022)

JACKS: joint analysis of CRISPR/Cas9 knockout screens
por: Allen, Felicity, et al.
Publicado: (2019)

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
por: Amorim, João Pedro, et al.
Publicado: (2020)

Different Mechanisms Must Be Considered to Explain the Increase in Hippocampal Neural Precursor Cell Proliferation by Physical Activity
por: Overall, Rupert W., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_vet461qmr6uc4lvpnun8apl24u