Highly efficient and safe genome editing by CRISPR-Cas12a using CRISPR RNA with a ribosyl-2′-O-methylated uridinylate-rich 3′-overhang in mouse zygotes

The CRISPR-Cas12a system has been developed to harness highly specific genome editing in eukaryotic cells. Given the relatively small sizes of Cas12a genes, the system has been suggested to be most applicable to gene therapy using AAV vector delivery. Previously, we reported that a U-rich crRNA enab...

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Detalles Bibliográficos
Autores principales: Ha, Dae-In, Lee, Jeong Mi, Lee, Nan-Ee, Kim, Daesik, Ko, Jeong-Heon, Kim, Yong-Sam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080787/
https://www.ncbi.nlm.nih.gov/pubmed/33162553
http://dx.doi.org/10.1038/s12276-020-00521-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8080787/
https://www.ncbi.nlm.nih.gov/pubmed/33162553
http://dx.doi.org/10.1038/s12276-020-00521-7

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