Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells

BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. T...

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Detalles Bibliográficos
Autores principales: Chen, Demeng, Zhang, Wuchang, Jia, Lingfei, Wang, Cheng, Wang, Cun-Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081985/
https://www.ncbi.nlm.nih.gov/pubmed/33982017
http://dx.doi.org/10.1016/j.xpro.2021.100484
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author Chen, Demeng
Zhang, Wuchang
Jia, Lingfei
Wang, Cheng
Wang, Cun-Yu
author_facet Chen, Demeng
Zhang, Wuchang
Jia, Lingfei
Wang, Cheng
Wang, Cun-Yu
author_sort Chen, Demeng
collection PubMed
description BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the Cre-loxP recombination strategy, which allows us to study the regulation and targeting of BMI1(+) CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020)
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spelling pubmed-80819852021-05-11 Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells Chen, Demeng Zhang, Wuchang Jia, Lingfei Wang, Cheng Wang, Cun-Yu STAR Protoc Protocol BMI1-expressing cancer stem cells (CSCs) play a key role in the development, progression, therapy resistance, recurrence, and metastasis of head and neck squamous cell carcinoma (HNSCC). Here, we present a chemically-induced HNSCC mouse model, genetically and pathologically similar to human HNSCC. This protocol describes how to use genetic lineage tracing based on the Cre-loxP recombination strategy, which allows us to study the regulation and targeting of BMI1(+) CSCs in primary tumors and lymph node metastases. For complete details on the use and execution of this protocol, please refer to Chen et al. (2017) and Jia et al. (2020) Elsevier 2021-04-18 /pmc/articles/PMC8081985/ /pubmed/33982017 http://dx.doi.org/10.1016/j.xpro.2021.100484 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Demeng
Zhang, Wuchang
Jia, Lingfei
Wang, Cheng
Wang, Cun-Yu
Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title_full Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title_fullStr Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title_full_unstemmed Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title_short Generation of a squamous cell carcinoma mouse model for lineage tracing of BMI1+ cancer stem cells
title_sort generation of a squamous cell carcinoma mouse model for lineage tracing of bmi1+ cancer stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8081985/
https://www.ncbi.nlm.nih.gov/pubmed/33982017
http://dx.doi.org/10.1016/j.xpro.2021.100484
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