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Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors

Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorti...

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Detalles Bibliográficos
Autores principales: Bieniasz-Krzywiec, Pawel, Martín-Pérez, Rosa, Riera-Domingo, Carla, Mazzone, Massimiliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082159/
https://www.ncbi.nlm.nih.gov/pubmed/33982015
http://dx.doi.org/10.1016/j.xpro.2021.100481