Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors

Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorti...

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Autores principales: Bieniasz-Krzywiec, Pawel, Martín-Pérez, Rosa, Riera-Domingo, Carla, Mazzone, Massimiliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082159/
https://www.ncbi.nlm.nih.gov/pubmed/33982015
http://dx.doi.org/10.1016/j.xpro.2021.100481
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author Bieniasz-Krzywiec, Pawel
Martín-Pérez, Rosa
Riera-Domingo, Carla
Mazzone, Massimiliano
author_facet Bieniasz-Krzywiec, Pawel
Martín-Pérez, Rosa
Riera-Domingo, Carla
Mazzone, Massimiliano
author_sort Bieniasz-Krzywiec, Pawel
collection PubMed
description Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorting (FACS) of TAMs from murine orthotopic 4T1 breast tumors. This includes steps to separate PoEMs (podoplanin-expressing macrophages) and non-PoEMs (podoplanin-negative macrophages). Our FACS separation approach could also be used for other tumor types with TAM infiltration. For complete details on the use and execution of this protocol, please refer to Bieniasz-Krzywiec et al. (2019).
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spelling pubmed-80821592021-05-11 Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors Bieniasz-Krzywiec, Pawel Martín-Pérez, Rosa Riera-Domingo, Carla Mazzone, Massimiliano STAR Protoc Protocol Tumor-associated macrophages (TAMs) are highly heterogenous regarding their intratumoral localization, surface marker expression, and molecular properties. This protocol describes the complete procedure for isolation and digestion of murine breast cancer samples and fluorescence-activated cell sorting (FACS) of TAMs from murine orthotopic 4T1 breast tumors. This includes steps to separate PoEMs (podoplanin-expressing macrophages) and non-PoEMs (podoplanin-negative macrophages). Our FACS separation approach could also be used for other tumor types with TAM infiltration. For complete details on the use and execution of this protocol, please refer to Bieniasz-Krzywiec et al. (2019). Elsevier 2021-04-17 /pmc/articles/PMC8082159/ /pubmed/33982015 http://dx.doi.org/10.1016/j.xpro.2021.100481 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bieniasz-Krzywiec, Pawel
Martín-Pérez, Rosa
Riera-Domingo, Carla
Mazzone, Massimiliano
Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title_full Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title_fullStr Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title_full_unstemmed Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title_short Isolation and separation of murine tumor-associated macrophages (TAMs) subpopulations from orthotopic 4T1 breast tumors
title_sort isolation and separation of murine tumor-associated macrophages (tams) subpopulations from orthotopic 4t1 breast tumors
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8082159/
https://www.ncbi.nlm.nih.gov/pubmed/33982015
http://dx.doi.org/10.1016/j.xpro.2021.100481
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