A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli

CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes; nevertheless, their function and application for genome en...

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Detalles Bibliográficos
Autores principales: Jervis, Adrian J., Hanko, Erik K.R., Dunstan, Mark S., Robinson, Christopher J., Takano, Eriko, Scrutton, Nigel S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085919/
https://www.ncbi.nlm.nih.gov/pubmed/33710766
http://dx.doi.org/10.1111/1751-7915.13780

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085919/
https://www.ncbi.nlm.nih.gov/pubmed/33710766
http://dx.doi.org/10.1111/1751-7915.13780

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