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A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli
CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes; nevertheless, their function and application for genome en...
Autores principales: | Jervis, Adrian J., Hanko, Erik K.R., Dunstan, Mark S., Robinson, Christopher J., Takano, Eriko, Scrutton, Nigel S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8085919/ https://www.ncbi.nlm.nih.gov/pubmed/33710766 http://dx.doi.org/10.1111/1751-7915.13780 |
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