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Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays

This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells...

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Detalles Bibliográficos
Autores principales: Tan, Lay Shuen, Chew, Resilind Su Ern, Ng, Natasha Hui Jin, Teo, Adrian Kee Keong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086138/
https://www.ncbi.nlm.nih.gov/pubmed/33997805
http://dx.doi.org/10.1016/j.xpro.2021.100471