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Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assays
This protocol describes the detailed procedures for utilizing human pluripotent stem cells (hPSCs) for pancreatic progenitor and hepatic differentiation, followed by the application of hPSC-derived cells in a luciferase reporter-based assay to study gene regulation. The generated hPSC-derived cells...
Autores principales: | Tan, Lay Shuen, Chew, Resilind Su Ern, Ng, Natasha Hui Jin, Teo, Adrian Kee Keong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086138/ https://www.ncbi.nlm.nih.gov/pubmed/33997805 http://dx.doi.org/10.1016/j.xpro.2021.100471 |
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