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Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme
This structural and biophysical study exploited a method of perdeuterating hen egg-white lysozyme based on the expression of insoluble protein in Escherichia coli followed by in-column chemical refolding. This allowed detailed comparisons with perdeuterated lysozyme produced in the yeast Pichia past...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086161/ https://www.ncbi.nlm.nih.gov/pubmed/33953924 http://dx.doi.org/10.1107/S2052252521001299 |