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Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme

This structural and biophysical study exploited a method of perdeuterating hen egg-white lysozyme based on the expression of insoluble protein in Escherichia coli followed by in-column chemical refolding. This allowed detailed comparisons with perdeuterated lysozyme produced in the yeast Pichia past...

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Detalles Bibliográficos
Autores principales:	Ramos, Joao, Laux, Valerie, Haertlein, Michael, Boeri Erba, Elisabetta, McAuley, Katherine E., Forsyth, V. Trevor, Mossou, Estelle, Larsen, Sine, Langkilde, Annette E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	International Union of Crystallography 2021
Materias:	Research Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086161/ https://www.ncbi.nlm.nih.gov/pubmed/33953924 http://dx.doi.org/10.1107/S2052252521001299

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