Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord

In vertebrates, motor control relies on cholinergic neurons in the spinal cord that have been extensively studied over the past hundred years, yet the full heterogeneity of these neurons and their different functional roles in the adult remain to be defined. Here, we develop a targeted single nuclea...

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Autores principales: Alkaslasi, Mor R., Piccus, Zoe E., Hareendran, Sangeetha, Silberberg, Hanna, Chen, Li, Zhang, Yajun, Petros, Timothy J., Le Pichon, Claire E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087807/
https://www.ncbi.nlm.nih.gov/pubmed/33931636
http://dx.doi.org/10.1038/s41467-021-22691-2
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author Alkaslasi, Mor R.
Piccus, Zoe E.
Hareendran, Sangeetha
Silberberg, Hanna
Chen, Li
Zhang, Yajun
Petros, Timothy J.
Le Pichon, Claire E.
author_facet Alkaslasi, Mor R.
Piccus, Zoe E.
Hareendran, Sangeetha
Silberberg, Hanna
Chen, Li
Zhang, Yajun
Petros, Timothy J.
Le Pichon, Claire E.
author_sort Alkaslasi, Mor R.
collection PubMed
description In vertebrates, motor control relies on cholinergic neurons in the spinal cord that have been extensively studied over the past hundred years, yet the full heterogeneity of these neurons and their different functional roles in the adult remain to be defined. Here, we develop a targeted single nuclear RNA sequencing approach and use it to identify an array of cholinergic interneurons, visceral and skeletal motor neurons. Our data expose markers for distinguishing these classes of cholinergic neurons and their rich diversity. Specifically, visceral motor neurons, which provide autonomic control, can be divided into more than a dozen transcriptomic classes with anatomically restricted localization along the spinal cord. The complexity of the skeletal motor neurons is also reflected in our analysis with alpha, gamma, and a third subtype, possibly corresponding to the elusive beta motor neurons, clearly distinguished. In combination, our data provide a comprehensive transcriptomic description of this important population of neurons that control many aspects of physiology and movement and encompass the cellular substrates for debilitating degenerative disorders.
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spelling pubmed-80878072021-05-11 Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord Alkaslasi, Mor R. Piccus, Zoe E. Hareendran, Sangeetha Silberberg, Hanna Chen, Li Zhang, Yajun Petros, Timothy J. Le Pichon, Claire E. Nat Commun Article In vertebrates, motor control relies on cholinergic neurons in the spinal cord that have been extensively studied over the past hundred years, yet the full heterogeneity of these neurons and their different functional roles in the adult remain to be defined. Here, we develop a targeted single nuclear RNA sequencing approach and use it to identify an array of cholinergic interneurons, visceral and skeletal motor neurons. Our data expose markers for distinguishing these classes of cholinergic neurons and their rich diversity. Specifically, visceral motor neurons, which provide autonomic control, can be divided into more than a dozen transcriptomic classes with anatomically restricted localization along the spinal cord. The complexity of the skeletal motor neurons is also reflected in our analysis with alpha, gamma, and a third subtype, possibly corresponding to the elusive beta motor neurons, clearly distinguished. In combination, our data provide a comprehensive transcriptomic description of this important population of neurons that control many aspects of physiology and movement and encompass the cellular substrates for debilitating degenerative disorders. Nature Publishing Group UK 2021-04-30 /pmc/articles/PMC8087807/ /pubmed/33931636 http://dx.doi.org/10.1038/s41467-021-22691-2 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Alkaslasi, Mor R.
Piccus, Zoe E.
Hareendran, Sangeetha
Silberberg, Hanna
Chen, Li
Zhang, Yajun
Petros, Timothy J.
Le Pichon, Claire E.
Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title_full Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title_fullStr Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title_full_unstemmed Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title_short Single nucleus RNA-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
title_sort single nucleus rna-sequencing defines unexpected diversity of cholinergic neuron types in the adult mouse spinal cord
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8087807/
https://www.ncbi.nlm.nih.gov/pubmed/33931636
http://dx.doi.org/10.1038/s41467-021-22691-2
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