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Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single‐cell level

Liquid biopsy analyzes the current status of primary tumors and their metastatic regions. We aimed to develop an optimized protocol for single‐cell sequencing of floating tumor cells (FTCs) in pleural effusion as a laboratory test. FTCs were enriched using a negative selection of white blood cells b...

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Detalles Bibliográficos
Autores principales: Nakamura, Ikuko Takeda, Ikegami, Masachika, Hasegawa, Nobuhiko, Hayashi, Takuo, Ueno, Toshihide, Kawazu, Masahito, Yagishita, Shigehiro, Goto, Yasushi, Shinno, Yuki, Kojima, Yuki, Takamochi, Kazuya, Takahashi, Fumiyuki, Takahashi, Kazuhisa, Mano, Hiroyuki, Kohsaka, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088920/
https://www.ncbi.nlm.nih.gov/pubmed/33484069
http://dx.doi.org/10.1111/cas.14821