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Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single‐cell level
Liquid biopsy analyzes the current status of primary tumors and their metastatic regions. We aimed to develop an optimized protocol for single‐cell sequencing of floating tumor cells (FTCs) in pleural effusion as a laboratory test. FTCs were enriched using a negative selection of white blood cells b...
Autores principales: | Nakamura, Ikuko Takeda, Ikegami, Masachika, Hasegawa, Nobuhiko, Hayashi, Takuo, Ueno, Toshihide, Kawazu, Masahito, Yagishita, Shigehiro, Goto, Yasushi, Shinno, Yuki, Kojima, Yuki, Takamochi, Kazuya, Takahashi, Fumiyuki, Takahashi, Kazuhisa, Mano, Hiroyuki, Kohsaka, Shinji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8088920/ https://www.ncbi.nlm.nih.gov/pubmed/33484069 http://dx.doi.org/10.1111/cas.14821 |
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