Optimization and mechanism exploration for Escherichia coli transformed with plasmid pUC19 by the combination with ultrasound treatment and chemical method

As a basic technique of molecular cloning, bio-transformation has been successfully used in the fields of biomedicine and food processing. In this study, we established a transformation system of exogenous DNA into E. coli cells mediated by ultrasound. Under the optimal conditions (i.e. 35 °C, 40 W,...

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Detalles Bibliográficos
Autores principales: Wang, Yonggang, Sun, Shangchen, Yu, Linmiao, Hu, Shu, Fan, Wenguang, Leng, Feifan, Ma, Jianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Original Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091046/
https://www.ncbi.nlm.nih.gov/pubmed/33887660
http://dx.doi.org/10.1016/j.ultsonch.2021.105552

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091046/
https://www.ncbi.nlm.nih.gov/pubmed/33887660
http://dx.doi.org/10.1016/j.ultsonch.2021.105552

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