Improvement of Opal Multiplex Immunofluorescence Workflow for Human Tissue Sections

The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluoropho...

Descripción completa

Detalles Bibliográficos
Autores principales: Willemsen, Marcella, Krebbers, Gabrielle, Bekkenk, Marcel W., Teunissen, Marcel B.M., Luiten, Rosalie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091416/
https://www.ncbi.nlm.nih.gov/pubmed/33797290
http://dx.doi.org/10.1369/00221554211007793

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091416/
https://www.ncbi.nlm.nih.gov/pubmed/33797290
http://dx.doi.org/10.1369/00221554211007793

Ejemplares similares