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Improvement of Opal Multiplex Immunofluorescence Workflow for Human Tissue Sections

The Opal multiplex technique is an established methodology for the detection of multiple biomarkers in one section. The protocol encompasses iterative single stainings and heating-mediated removal of the primary and secondary antibodies after each staining round, leaving untouched the Opal fluoropho...

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Detalles Bibliográficos
Autores principales:	Willemsen, Marcella, Krebbers, Gabrielle, Bekkenk, Marcel W., Teunissen, Marcel B.M., Luiten, Rosalie M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	SAGE Publications 2021
Materias:	Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091416/ https://www.ncbi.nlm.nih.gov/pubmed/33797290 http://dx.doi.org/10.1369/00221554211007793

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