Rapid High-Throughput Whole-Genome Sequencing of SARS-CoV-2 by Using One-Step Reverse Transcription-PCR Amplification with an Integrated Microfluidic System and Next-Generation Sequencing

The long-lasting global COVID-19 pandemic demands timely genomic investigation of SARS-CoV-2 viruses. Here, we report a simple and efficient workflow for whole-genome sequencing utilizing one-step reverse transcription-PCR (RT-PCR) amplification on a microfluidic platform, followed by MiSeq amplicon...

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Detalles Bibliográficos
Autores principales: Li, Tao, Chung, Hye Kyung, Pireku, Papa K., Beitzel, Brett F., Sanborn, Mark A., Tang, Cynthia Y., Hammer, Richard D., Ritter, Detlef, Wan, Xiu-Feng, Maljkovic Berry, Irina, Hang, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091833/
https://www.ncbi.nlm.nih.gov/pubmed/33653700
http://dx.doi.org/10.1128/JCM.02784-20

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091833/
https://www.ncbi.nlm.nih.gov/pubmed/33653700
http://dx.doi.org/10.1128/JCM.02784-20

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