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Measurement of labile and protein-bound heme in fixed prostate cancer cells and in cellular fractions
Labile heme is present in the cells at very low concentrations, either unbound or loosely bound to molecules, and accessible for signaling as alarmin. Our recent work suggests that extracellular heme can be taken up and detected in the nuclei of cancer cells. Here, we describe the detailed protocol...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8091920/ https://www.ncbi.nlm.nih.gov/pubmed/33997811 http://dx.doi.org/10.1016/j.xpro.2021.100491 |