A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor

Targeted mutagenesis in model organisms is key for gene functional annotation and biomedical research. Despite technological advances in gene editing by the CRISPR-Cas9 systems, rapid and efficient introduction of site-directed mutations remains a challenge in large animal models. Here, we developed...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Hengxing, Liu, Jun, Feng, Tao, Guo, Zihang, Yin, Yunjun, Gao, Fei, Cao, Gengsheng, Du, Xuguang, Wu, Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092013/
https://www.ncbi.nlm.nih.gov/pubmed/33795333
http://dx.doi.org/10.1101/gr.271312.120

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8092013/
https://www.ncbi.nlm.nih.gov/pubmed/33795333
http://dx.doi.org/10.1101/gr.271312.120

Ejemplares similares