Cargando…
Nearest-neighbour transition-state analysis for nucleic acid kinetics
We used stopped-flow to monitor hypochromicity for 43 oligonucleotide duplexes to study nucleic acid kinetics and extract transition-state parameters for association and dissociation. Reactions were performed in 1.0 M NaCl (for literature comparisons) and 2.2 mM MgCl(2) (PCR conditions). Dissociatio...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096236/ https://www.ncbi.nlm.nih.gov/pubmed/33823552 http://dx.doi.org/10.1093/nar/gkab205 |