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Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner

Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an...

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Autores principales: Hamad, Nesreen, Yoneda, Ryoma, So, Masatomo, Kurokawa, Riki, Nagata, Takashi, Katahira, Masato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096841/
https://www.ncbi.nlm.nih.gov/pubmed/33947944
http://dx.doi.org/10.1038/s41598-021-89075-w
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author Hamad, Nesreen
Yoneda, Ryoma
So, Masatomo
Kurokawa, Riki
Nagata, Takashi
Katahira, Masato
author_facet Hamad, Nesreen
Yoneda, Ryoma
So, Masatomo
Kurokawa, Riki
Nagata, Takashi
Katahira, Masato
author_sort Hamad, Nesreen
collection PubMed
description Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases.
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spelling pubmed-80968412021-05-05 Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner Hamad, Nesreen Yoneda, Ryoma So, Masatomo Kurokawa, Riki Nagata, Takashi Katahira, Masato Sci Rep Article Fused in sarcoma/translocated in liposarcoma (FUS/TLS) is a multitasking RNA/DNA binding protein. FUS aggregation is implicated in various neurodegenerative diseases. RNA was suggested to modulate phase transition of FUS. Here, we found that FUS transforms into the amorphous aggregation state as an instant response to the shear stress caused by usual pipetting even at a low FUS concentration, 100 nM. It was revealed that non-coding RNA can suppress the transformation of FUS into aggregates. The suppressive effect of RNA on FUS aggregation is sequence-dependent. These results suggested that the non-coding RNA could be a prospective suppressor of FUS aggregation caused by mechanistic stress in cells. Our finding might pave the way for more research on the role of RNAs as aggregation inhibitors, which could facilitate the development of therapies for neurodegenerative diseases. Nature Publishing Group UK 2021-05-04 /pmc/articles/PMC8096841/ /pubmed/33947944 http://dx.doi.org/10.1038/s41598-021-89075-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hamad, Nesreen
Yoneda, Ryoma
So, Masatomo
Kurokawa, Riki
Nagata, Takashi
Katahira, Masato
Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_full Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_fullStr Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_full_unstemmed Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_short Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
title_sort non-coding rna suppresses fus aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8096841/
https://www.ncbi.nlm.nih.gov/pubmed/33947944
http://dx.doi.org/10.1038/s41598-021-89075-w
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